In vitro susceptibility tests of dermatophytes to griseofulvin and imidazole derivatives
The sensitivity of dermatophytes (89 'wild' and 38 'collections' strains) to griseofulvin and azole derivatives (clotrimazole, cetoconazole, tioconazole, miconazole, econazole, isoconazole and oxiconazole) was studied using 'in vitro' determinations of minimum inhibitory concentration (MIC) and mini...
| Autor principal: | Souza Furtado, Maria do Socorro de |
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| Outros Autores: | Minami, Paulo Suyoshi |
| Grau: | Artigo |
| Idioma: | pt_BR |
| Publicado em: |
Revista de Microbiologia
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/19349 |
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oai:repositorio:1-19349 |
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oai:repositorio:1-19349 In vitro susceptibility tests of dermatophytes to griseofulvin and imidazole derivatives Testes de sensibilidade 'in vitro' de dermatofitos a grieseofulvina e derivados imidazolicos Souza Furtado, Maria do Socorro de Minami, Paulo Suyoshi Clotrimazole Econazole Griseofulvin Imidazole Derivative Isoconazole Ketoconazole Miconazole Oxiconazole Tioconazole Antibiotic Sensitivity Dermatophyte Epidermophyton Floccosum Fungicidal Activity Microsporum Microsporum Canis Microsporum Gypseum Minimum Inhibitory Concentration Nonhuman Trichophyton Trichophyton Mentagrophytes Trichophyton Rubrum The sensitivity of dermatophytes (89 'wild' and 38 'collections' strains) to griseofulvin and azole derivatives (clotrimazole, cetoconazole, tioconazole, miconazole, econazole, isoconazole and oxiconazole) was studied using 'in vitro' determinations of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The broth dilution method was used; the antifungal drugs were used in concentrations between 0,05 to 100 μg/ml. The inoculum was (standardized adjusting the suspension to contain) 1,0 to 1,5 X 106 ufc/ml. The MIC and MFC values showed different results for many fungi strains and different concentrations of antifungal agents. The values for clotrimazole varied from 0,1 to 1,56 μg/ml in almost all strains. Higher values were obtained for T. rubrum (12,5 μg/ml) and T. mentagrophytes (25,0 μg/ml). Cetoconazole was the imidazole drag that showed the highest MIC and MFC values, as high as 100,0 μg/ml when tested against the species of T. rubrum, T. mentagrophytes, T. tonsurans and M. gypseum. The antifungal activities of the other imidazole drugs were comparable, the MIC and MFC values varied from 0,10 to 12,5 μg/ml. In relation to griseofulvin, the fungi used in this study, presented two different behaviours: higher MIC and MFC values (12,5 to 100,0 μg/ml) for T. rubrum, T. mentagrophyte, T. tonsurans, T. soudanense, M. canis, M. gypseum and M. persicolor and lower values (0,39 to 3,12 μg/ml) for T. schoenleinii, T. violaceum, E. floccosum, and M. audouinii. E. floccosum, M. canis and T. schoenleinii had higher susceptibilitis to the drugs, (MIC and MFC values varying from 0,05 to 0,20 μg/ml), when compared to T. mentagrophytes, T. rubrum, M. gypseum and T. soudanense. No important differences were observed in file MIC and MFC values for the 'wild' and the 'collection' strains. 2020-06-15T22:07:41Z 2020-06-15T22:07:41Z 1997 Artigo https://repositorio.inpa.gov.br/handle/1/19349 pt_BR Volume 28, Número 2, Pags. 110-115 Restrito Revista de Microbiologia |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
pt_BR |
| topic |
Clotrimazole Econazole Griseofulvin Imidazole Derivative Isoconazole Ketoconazole Miconazole Oxiconazole Tioconazole Antibiotic Sensitivity Dermatophyte Epidermophyton Floccosum Fungicidal Activity Microsporum Microsporum Canis Microsporum Gypseum Minimum Inhibitory Concentration Nonhuman Trichophyton Trichophyton Mentagrophytes Trichophyton Rubrum |
| spellingShingle |
Clotrimazole Econazole Griseofulvin Imidazole Derivative Isoconazole Ketoconazole Miconazole Oxiconazole Tioconazole Antibiotic Sensitivity Dermatophyte Epidermophyton Floccosum Fungicidal Activity Microsporum Microsporum Canis Microsporum Gypseum Minimum Inhibitory Concentration Nonhuman Trichophyton Trichophyton Mentagrophytes Trichophyton Rubrum Souza Furtado, Maria do Socorro de In vitro susceptibility tests of dermatophytes to griseofulvin and imidazole derivatives |
| topic_facet |
Clotrimazole Econazole Griseofulvin Imidazole Derivative Isoconazole Ketoconazole Miconazole Oxiconazole Tioconazole Antibiotic Sensitivity Dermatophyte Epidermophyton Floccosum Fungicidal Activity Microsporum Microsporum Canis Microsporum Gypseum Minimum Inhibitory Concentration Nonhuman Trichophyton Trichophyton Mentagrophytes Trichophyton Rubrum |
| description |
The sensitivity of dermatophytes (89 'wild' and 38 'collections' strains) to griseofulvin and azole derivatives (clotrimazole, cetoconazole, tioconazole, miconazole, econazole, isoconazole and oxiconazole) was studied using 'in vitro' determinations of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The broth dilution method was used; the antifungal drugs were used in concentrations between 0,05 to 100 μg/ml. The inoculum was (standardized adjusting the suspension to contain) 1,0 to 1,5 X 106 ufc/ml. The MIC and MFC values showed different results for many fungi strains and different concentrations of antifungal agents. The values for clotrimazole varied from 0,1 to 1,56 μg/ml in almost all strains. Higher values were obtained for T. rubrum (12,5 μg/ml) and T. mentagrophytes (25,0 μg/ml). Cetoconazole was the imidazole drag that showed the highest MIC and MFC values, as high as 100,0 μg/ml when tested against the species of T. rubrum, T. mentagrophytes, T. tonsurans and M. gypseum. The antifungal activities of the other imidazole drugs were comparable, the MIC and MFC values varied from 0,10 to 12,5 μg/ml. In relation to griseofulvin, the fungi used in this study, presented two different behaviours: higher MIC and MFC values (12,5 to 100,0 μg/ml) for T. rubrum, T. mentagrophyte, T. tonsurans, T. soudanense, M. canis, M. gypseum and M. persicolor and lower values (0,39 to 3,12 μg/ml) for T. schoenleinii, T. violaceum, E. floccosum, and M. audouinii. E. floccosum, M. canis and T. schoenleinii had higher susceptibilitis to the drugs, (MIC and MFC values varying from 0,05 to 0,20 μg/ml), when compared to T. mentagrophytes, T. rubrum, M. gypseum and T. soudanense. No important differences were observed in file MIC and MFC values for the 'wild' and the 'collection' strains. |
| format |
Artigo |
| author |
Souza Furtado, Maria do Socorro de |
| author2 |
Minami, Paulo Suyoshi |
| author2Str |
Minami, Paulo Suyoshi |
| title |
In vitro susceptibility tests of dermatophytes to griseofulvin and imidazole derivatives |
| title_short |
In vitro susceptibility tests of dermatophytes to griseofulvin and imidazole derivatives |
| title_full |
In vitro susceptibility tests of dermatophytes to griseofulvin and imidazole derivatives |
| title_fullStr |
In vitro susceptibility tests of dermatophytes to griseofulvin and imidazole derivatives |
| title_full_unstemmed |
In vitro susceptibility tests of dermatophytes to griseofulvin and imidazole derivatives |
| title_sort |
in vitro susceptibility tests of dermatophytes to griseofulvin and imidazole derivatives |
| publisher |
Revista de Microbiologia |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/19349 |
| _version_ |
1787142614660677632 |
| score |
11.755432 |