Currently there has been an increased interest in the separation process of the individual whey
proteins from different adsorbents, this is due to its universal applicability. The need to use
adsorbents with lower costs in industrial production has been important for the separation
process. The bovine serum albumin (BSA) is a protein present in bovine milk whey and has
been studied as a function of their functional and nutritional qualities for biotechnological
utilization purposes. This work was developed to study the process of separation of this
protein at different pH (4.0 to 7.0), concentrations (0.0 to 7.0 mg g-1) and NaCl concentration
in the adsorptive process. For separation were used adsorption method, using commercial
hydroxyapatite (HA), synthetic hydroxyapatite (SAH) and activated carbon babassu (CAB).
Initially it was performed kinetic studies of Pseudo 1st order models and Pseudo 2nd order.
adsorptive efficiency of the process was also carried out. The characterization was done by
analysis of the zeta potential of the adsorbents studied. For isotherms, equilibrium data were
performed studies using Langmuir, Freundlich and Jovanovic. The effect of NaCl
concentration was evaluated without the use of salt and (1.0 mol L-1) to the Langmuir model.
The kinetic results were better represented in the Pseudo 2nd order model with chemisorption
characteristics. The efficiency was improved at pH 4.0 for HA with (74.3%), with
hypertension (68.7%) and CAB are obtained (37.3%). The fillers obtained from zeta potential
ranging from (-6.6 and -42,8mV). The results for pH 4.0 were better, and the maximum
adsorption capacity (qm): qm = 85.53 mg g-1 g-1 68.96 mg and 36.18 mg g-1 for HA, HAS and
CAB respectively. For the three adsorbents noted that (qm) BSA decreased with increasing
NaCl concentration. For the pH (4,0; 6,0 and 7,0), It was observed that the (qm) decreased as
the pH increased indicating that electrostatic bonds and functional groups on the surface of
adsorbents contributed to this reduction, particularly for the CAB, for a statistically significant
difference was found (p<0.05).
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