Separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (Orbignya martiana)
Currently there has been an increased interest in the separation process of the individual whey proteins from different adsorbents, this is due to its universal applicability. The need to use adsorbents with lower costs in industrial production has been important for the separation process. The b...
| Autor principal: | Alves, Márcia Regina Ribeiro |
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| Grau: | Tese |
| Idioma: | pt_BR |
| Publicado em: |
Universidade Federal do Tocantins
2016
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| Acesso em linha: |
http://hdl.handle.net/11612/209 |
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ir-11612-209 |
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ir-11612-2092019-05-25T06:04:57Z Separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (Orbignya martiana) Alves, Márcia Regina Ribeiro Zuñiga, Braham Damian Giraldo Sousa, Rita de Cássia Superbi de Adsorção Albumina de soro bovino Hidroxiapatita Babaçu Potencial zeta CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA::TECNOLOGIA QUIMICA::ALIMENTOS Currently there has been an increased interest in the separation process of the individual whey proteins from different adsorbents, this is due to its universal applicability. The need to use adsorbents with lower costs in industrial production has been important for the separation process. The bovine serum albumin (BSA) is a protein present in bovine milk whey and has been studied as a function of their functional and nutritional qualities for biotechnological utilization purposes. This work was developed to study the process of separation of this protein at different pH (4.0 to 7.0), concentrations (0.0 to 7.0 mg g-1) and NaCl concentration in the adsorptive process. For separation were used adsorption method, using commercial hydroxyapatite (HA), synthetic hydroxyapatite (SAH) and activated carbon babassu (CAB). Initially it was performed kinetic studies of Pseudo 1st order models and Pseudo 2nd order. adsorptive efficiency of the process was also carried out. The characterization was done by analysis of the zeta potential of the adsorbents studied. For isotherms, equilibrium data were performed studies using Langmuir, Freundlich and Jovanovic. The effect of NaCl concentration was evaluated without the use of salt and (1.0 mol L-1) to the Langmuir model. The kinetic results were better represented in the Pseudo 2nd order model with chemisorption characteristics. The efficiency was improved at pH 4.0 for HA with (74.3%), with hypertension (68.7%) and CAB are obtained (37.3%). The fillers obtained from zeta potential ranging from (-6.6 and -42,8mV). The results for pH 4.0 were better, and the maximum adsorption capacity (qm): qm = 85.53 mg g-1 g-1 68.96 mg and 36.18 mg g-1 for HA, HAS and CAB respectively. For the three adsorbents noted that (qm) BSA decreased with increasing NaCl concentration. For the pH (4,0; 6,0 and 7,0), It was observed that the (qm) decreased as the pH increased indicating that electrostatic bonds and functional groups on the surface of adsorbents contributed to this reduction, particularly for the CAB, for a statistically significant difference was found (p<0.05). Atualmente observa-se um aumento do interesse no processo de separação de proteínas individuais do soro de leite a partir de diferentes adsorventes devido à sua aplicabilidade universal. A necessidade do uso de adsorventes com custos mais baixos na produção industrial tem sido importante para os processos de separação. A albumina de soro bovino (BSA) é uma proteína presente no soro de leite bovino e tem sido estudada em função de suas qualidades funcionais e nutricionais para fins de aproveitamento biotecnológico. Este trabalho foi desenvolvido com o objetivo de estudar o processo de separação desta proteína em diferentes pH (4,0; 5,0; 6,0 e 7,0), concentrações (0,0 a 7,0 mg g-1) e concentração de NaCl no processo adsortivo. Para a separação foram utilizados método de adsorção, com utilização de hidroxiapatita comercial (HA), hidroxiapatita sintética (HAS) e carvão ativo de babaçu (CAB). Inicialmente foram realizados estudos cinéticos dos modelos de Pseudo 1ª ordem e Pseudo 2ª ordem. Foi também realizada a eficiência do processo adsortivo. A caracterização foi feita através de análises do potencial zeta nos adsorventes estudados. Para as isotermas, foram realizados estudos de dados de equilíbrio através de modelos de Langmuir, Freundlich e Jovanovic. O efeito da concentração de NaCl, foi avaliado sem utilização do sal e a (1,0 mol L-1) para o modelo de Langmuir. Os resultados cinéticos foi melhor representado no modelo de Pseudo 2ª ordem com características de quimissorção. A eficiência foi mais satisfatória em pH 4,0 para HA com 74,3 %, HAS com 68,7 %, e CAB sendo obtido 37,3%. As cargas obtidas de potencial zeta variaram entre (-6,6 e -42,8mV). Os resultados para o pH 4,0 foram melhores, sendo que, a capacidade máxima de adsorção (qm): qm = 85,53 mg g-1 , 68,96 mg g-1 e 36,18 mg g-1 para a HA, HAS e CAB, respectivamente. Para os três adsorventes foi observado que o qm de BSA diminuiu com o aumento da concentração de NaCl. Para os pH (4,0; 6,0 e 7,0) o (qm) diminuiu à medida que o pH aumentou indicando que ligações eletrostáticas e grupos funcionais da superfície dos adsorventes contribuíram para essa a redução, sobretudo para o CAB foi encontrada diferença estatística significativa (p<0.05). 2016-05-30T17:30:54Z 2016-05-30T17:30:54Z 2016-05-09 Tese ALVES, Márcia Regina Ribeiro. Separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (Orbignya martiana). 2016. 108f. Tese (Doutorado em Biodiversidade e Biotecnologia) – Universidade Federal do Tocantins, Programa de Pós-Graduação em Biodiversidade e Biotecnologia, Palmas, 2016. http://hdl.handle.net/11612/209 pt_BR Open Access application/pdf application/pdf Universidade Federal do Tocantins BR Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Amazônia Legal - Bionorte Palmas |
| institution |
Repositório Institucional - Universidade Federal do Tocantins - UFT |
| collection |
RepositorioUFT |
| language |
pt_BR |
| topic |
Adsorção Albumina de soro bovino Hidroxiapatita Babaçu Potencial zeta CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA::TECNOLOGIA QUIMICA::ALIMENTOS |
| spellingShingle |
Adsorção Albumina de soro bovino Hidroxiapatita Babaçu Potencial zeta CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA::TECNOLOGIA QUIMICA::ALIMENTOS Alves, Márcia Regina Ribeiro Separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (Orbignya martiana) |
| topic_facet |
Adsorção Albumina de soro bovino Hidroxiapatita Babaçu Potencial zeta CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA::TECNOLOGIA QUIMICA::ALIMENTOS |
| description |
Currently there has been an increased interest in the separation process of the individual whey
proteins from different adsorbents, this is due to its universal applicability. The need to use
adsorbents with lower costs in industrial production has been important for the separation
process. The bovine serum albumin (BSA) is a protein present in bovine milk whey and has
been studied as a function of their functional and nutritional qualities for biotechnological
utilization purposes. This work was developed to study the process of separation of this
protein at different pH (4.0 to 7.0), concentrations (0.0 to 7.0 mg g-1) and NaCl concentration
in the adsorptive process. For separation were used adsorption method, using commercial
hydroxyapatite (HA), synthetic hydroxyapatite (SAH) and activated carbon babassu (CAB).
Initially it was performed kinetic studies of Pseudo 1st order models and Pseudo 2nd order.
adsorptive efficiency of the process was also carried out. The characterization was done by
analysis of the zeta potential of the adsorbents studied. For isotherms, equilibrium data were
performed studies using Langmuir, Freundlich and Jovanovic. The effect of NaCl
concentration was evaluated without the use of salt and (1.0 mol L-1) to the Langmuir model.
The kinetic results were better represented in the Pseudo 2nd order model with chemisorption
characteristics. The efficiency was improved at pH 4.0 for HA with (74.3%), with
hypertension (68.7%) and CAB are obtained (37.3%). The fillers obtained from zeta potential
ranging from (-6.6 and -42,8mV). The results for pH 4.0 were better, and the maximum
adsorption capacity (qm): qm = 85.53 mg g-1 g-1 68.96 mg and 36.18 mg g-1 for HA, HAS and
CAB respectively. For the three adsorbents noted that (qm) BSA decreased with increasing
NaCl concentration. For the pH (4,0; 6,0 and 7,0), It was observed that the (qm) decreased as
the pH increased indicating that electrostatic bonds and functional groups on the surface of
adsorbents contributed to this reduction, particularly for the CAB, for a statistically significant
difference was found (p<0.05). |
| author_additional |
Zuñiga, Braham Damian Giraldo |
| author_additionalStr |
Zuñiga, Braham Damian Giraldo |
| format |
Tese |
| author |
Alves, Márcia Regina Ribeiro |
| title |
Separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (Orbignya martiana) |
| title_short |
Separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (Orbignya martiana) |
| title_full |
Separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (Orbignya martiana) |
| title_fullStr |
Separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (Orbignya martiana) |
| title_full_unstemmed |
Separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (Orbignya martiana) |
| title_sort |
separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (orbignya martiana) |
| publisher |
Universidade Federal do Tocantins |
| publishDate |
2016 |
| url |
http://hdl.handle.net/11612/209 |
| _version_ |
1787134941782343680 |
| score |
11.755432 |