Platonia insignis Mart. is a species belonging to Clusiaceae, is popularly known as Bacuri and is well-known for having a fruit of appreciated flavor. The ethnopharmacological use is related to the use of the oil of its seeds in the healing of scars and as an anti-inflammatory and in the production of soap. Currently, several activities have been reported for all parts of this fruit (seed, bark and pulp). This work aimed to analyze the phytochemical profile, biological, and antimicrobial activity of the leaf extract of Platonia insignis Mart., and develop pharmaceutical formulations. Phytochemical analysis of the extract showed the presence of phenols, condensed tannins, catechins, steroids, alkaloids, flavonoids and saponins. Analyzes by LC-MS and FIA-ESI-IT/MSn provided the fragmentation profiles of the compounds present in the extract and their structures were proposed. In the ethyl acetate fraction, fukugentin (morelloflavone) was identified as an important marker of the clusiaceae family. The extract presented antioxidant potential in the inhibition of 88,06% of the DPPH radical. The soap and the topical solution manipulated from the 1 and 5% extract had their stability analyzed at time zero and thirty days after formulations, besides the evaluation of the microbiological quality control. The antimicrobial activity was evaluated by the microdilution technique. The hydroethanolic extract (70%), the hexane, ethyl acetate and the manipulated products presented antimicrobial activity with MIC between 0.78 and 12.5 mg/mL to all the microorganisms tested except the organic fractions (ethyl acetate and hexane) that showed no activity against Acinetobacter baumannii. The toxicity evaluated by the hemolysis technique did not demonstrate hemolytic activity up to a concentration of 100 mg/mL. Toxicity to Artemia salina showed that the extract had LD50 of 42.6 μg/mL being classified as highly toxic against Artemia salina. The extract has antioxidant activity, microbicide and low in vitro toxicity. Pharmaceutical formulations have proved potentially viable in the development of novel products for the treatment of infectious diseases.
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