In the carcinogenesis of gastric cancer, the stages usually manifest clinically as gastritis,
gastric atrophy, ulcerations, intestinal metaplasia, dysplasia and finally, as malignant neoplasia.
The association between gastric cancer and diet is already widely described in the literature and
several studies have demonstrated the influence of food intake with preservatives and with high
concentration of nitrates and salt, with the development of this neoplasia. Regarding water
consumption, there’s no relevant evidences. The pH of most of the water sold in the
metropolitan área of Belem does not match the standarts recommended by the govermnment
Health department, being more acidic. Thus, the benefits of both healthy eating and alkaline
water consumption are object of several discussions. Nowadays the other types of markers that
can aid the detection of pre-neoplastic and neoplastic lesions will be hosted. Among them, find
themselves as epigenetic proteins. Environmental factors such as diet, inflammation and
infection have been excluded as contributors to epigenetic changes. Hence, the present work
intends to provide evidence that only the water pH modification is able to lead the variations
the expressions pattern of miRNAs, associated with a first stage of gastric carcinogenesis, a
gastritis. For this it was applied the microRNAs miR-7, mir-155, mir-29c and mir-135b, in 28
patients porters of gastritis that were burned to digestive endoscopy alkaline PH. After
collection, the RNA from the samples was extracted, and the complementary tape DNA
(cDNA) was obtained. The cDNAs were submitted to qPCR amplification analysis for analysis
of miRNA expression. They assessment were using the Biostat and Stata 11.0 programs, being
statistically superior to values of p <0.05. Comparing the levels of expression and clinical
evaluation of gastritis by EDA before and after alkaline water consumption, the results
demonstrated that there was a increasing of the target microRNAs, of miR-7 (p = 0.09), miR155 (p = 0.13), miR-29c (p =0.21) and miR-135b(p=0.19). On the other hand, it was possible
to observe a significant endoscopic improvement of the gastritis (p=0.024), demonstrating the
clinical benefit of alkaline water intake.
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