The main method of controlling the cattle tick Boophilus microplus is the use of chemical acaricides during the parasite phase. However, the intense use of this method is causing economic
damage and promoting the rapid selection of resistant ticks. Besides the risk of residues in milk,
in meat and environmental contamination. Within this context, the use of herbal points out as an
alternative to control the tick and can reduce the economic and environmental impacts of the use
of synthetic chemicals. On this way, the purpose of this study was to evaluate "in vitro" the effect
of essential oils of grass-salsa (Cymbopogon winteranius Jowitt) and priprioca (Cyperus
articulatus L.), chemicals isolated substances from timbó (Derris nicou; Derris urucu): rotenone,
rotenolone and degueline and Andiroba’s oil (Carapa guianensis Aublet.) and neem (Azadirachta
indica A. Juss.) in the control of ticks. The searches were conducted on the premises of the
Animal Research Unit "Sen. Álvaro Adolpho, of "the Brazilian Enterprise for Agricultural
Research - Embrapa Eastern Amazon, in the city of Belém, state of Pará, in the period from
March to July of 2008. The engorged females of Boophilus microplus were collected manually,
from naturally infected cattle on private property located within an hour of Belém. After
collection, they were transported to the laboratory in plastic containers, marked, cleaned and
aerated. In the laboratory, they were rinsed in running water, dried on absorbent paper and
weighed on analytical balance (accuracy of 0.0001 g). The methodology used was the immersion
of teleogines by time of five minutes. After soaking, the ticks were dried on absorbent paper, set
in petri dishes and kept at room temperature for completion of the posture. Throughout the trial
period the temperature and relative humidity was measured in the laboratory using a
termohigrometer. The experimental design used was completely randomized. The percentage of
mortality of engorged females of B. microplus, on 5 and 15, varied: 45% to 90%, 37% to 90% and
60 to 90%, 60% to 90% for grass-salsa and priprioca respectively. The results for the stance
showed no statistical difference between the concentrations 2% and 10% on the control (water +
ethanol). However, there was a difference in the concentration 50% for both grass-salsa as
priprioca. For grass-salsa, this concentration was observed egg laying infertile. Regarding the
percentage of efficiency of the product, the values obtained were: 100%; 95.63%, 98.77%, 100%
and 99,30%, 96,97%, 99,85% and 100% to the effects of essential oil of salsa and grass-priprioca
concentrations 2%, 5%, 10%, 50%, respectively. Mortality rates, on the 5th , ranged from 37% to
41%, 49% to 64% and 52% to 41% for rotenone, rotenolone and degueline from low to high concentration, respectively. All substances tested showed significantly higher mortality rate than
those obtained by the control group (water + ethanol). The best results were posture of percentage
of 49% and 26% for the rotenone degueline and 200ppm, respectively and 34% for rotenolone at
100ppm. The percentage of efficiency in tests with rotenone were 95.24% and 98.68% for
rotenolone the order of 97.29%, 98.08%, 100% and degueline 95.45%, 98.23% and 100 %, from
100ppm, 200ppm and 300ppm, respectively. To andiroba and neem, the percentage of mortality
was observed on 15 and has a natural behavior of the life cycle of ticks because there was no
statistical difference between the control (water + ethanol) and treatments in different
concentrations. To andiroba and neem the higher efficiency obtained was 98,01% and 100%, at
concentration 50%, respectively.
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