Prospecção de biocida em plantas amazônicas e exóticas, visando seu uso racional
The main method of controlling the cattle tick Boophilus microplus is the use of chemical acaricides during the parasite phase. However, the intense use of this method is causing economic damage and promoting the rapid selection of resistant ticks. Besides the risk of residues in milk, in meat and...
| Autor principal: | DANTAS, Eneida Pontes Mota |
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| Grau: | Dissertação |
| Idioma: | por |
| Publicado em: |
Universidade Federal do Pará
2014
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| Acesso em linha: |
http://repositorio.ufpa.br/jspui/handle/2011/4713 |
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ir-2011-4713 |
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ir-2011-47132019-05-22T15:59:54Z Prospecção de biocida em plantas amazônicas e exóticas, visando seu uso racional DANTAS, Eneida Pontes Mota SOUZA FILHO, Antonio Pedro da Silva http://lattes.cnpq.br/1691897760012496 Ruminantes Bovino Carrapato bovino Boophilus microplus Atividade biocida Controle biológico Praga Óleo aromático Belém - PA Pará - Estado Amazônia brasileira CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA The main method of controlling the cattle tick Boophilus microplus is the use of chemical acaricides during the parasite phase. However, the intense use of this method is causing economic damage and promoting the rapid selection of resistant ticks. Besides the risk of residues in milk, in meat and environmental contamination. Within this context, the use of herbal points out as an alternative to control the tick and can reduce the economic and environmental impacts of the use of synthetic chemicals. On this way, the purpose of this study was to evaluate "in vitro" the effect of essential oils of grass-salsa (Cymbopogon winteranius Jowitt) and priprioca (Cyperus articulatus L.), chemicals isolated substances from timbó (Derris nicou; Derris urucu): rotenone, rotenolone and degueline and Andiroba’s oil (Carapa guianensis Aublet.) and neem (Azadirachta indica A. Juss.) in the control of ticks. The searches were conducted on the premises of the Animal Research Unit "Sen. Álvaro Adolpho, of "the Brazilian Enterprise for Agricultural Research - Embrapa Eastern Amazon, in the city of Belém, state of Pará, in the period from March to July of 2008. The engorged females of Boophilus microplus were collected manually, from naturally infected cattle on private property located within an hour of Belém. After collection, they were transported to the laboratory in plastic containers, marked, cleaned and aerated. In the laboratory, they were rinsed in running water, dried on absorbent paper and weighed on analytical balance (accuracy of 0.0001 g). The methodology used was the immersion of teleogines by time of five minutes. After soaking, the ticks were dried on absorbent paper, set in petri dishes and kept at room temperature for completion of the posture. Throughout the trial period the temperature and relative humidity was measured in the laboratory using a termohigrometer. The experimental design used was completely randomized. The percentage of mortality of engorged females of B. microplus, on 5 and 15, varied: 45% to 90%, 37% to 90% and 60 to 90%, 60% to 90% for grass-salsa and priprioca respectively. The results for the stance showed no statistical difference between the concentrations 2% and 10% on the control (water + ethanol). However, there was a difference in the concentration 50% for both grass-salsa as priprioca. For grass-salsa, this concentration was observed egg laying infertile. Regarding the percentage of efficiency of the product, the values obtained were: 100%; 95.63%, 98.77%, 100% and 99,30%, 96,97%, 99,85% and 100% to the effects of essential oil of salsa and grass-priprioca concentrations 2%, 5%, 10%, 50%, respectively. Mortality rates, on the 5th , ranged from 37% to 41%, 49% to 64% and 52% to 41% for rotenone, rotenolone and degueline from low to high concentration, respectively. All substances tested showed significantly higher mortality rate than those obtained by the control group (water + ethanol). The best results were posture of percentage of 49% and 26% for the rotenone degueline and 200ppm, respectively and 34% for rotenolone at 100ppm. The percentage of efficiency in tests with rotenone were 95.24% and 98.68% for rotenolone the order of 97.29%, 98.08%, 100% and degueline 95.45%, 98.23% and 100 %, from 100ppm, 200ppm and 300ppm, respectively. To andiroba and neem, the percentage of mortality was observed on 15 and has a natural behavior of the life cycle of ticks because there was no statistical difference between the control (water + ethanol) and treatments in different concentrations. To andiroba and neem the higher efficiency obtained was 98,01% and 100%, at concentration 50%, respectively. CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico PPG7 - Programa Piloto para a Proteção das Florestas Tropicais do Brasil O principal método de controle do carrapato bovino Boophilus microplus é o uso de carrapaticidas sintéticos. Entretanto, o rápido desenvolvimento de resistência ao princípio ativo, os riscos de resíduo nos produtos de origem animal e a contaminação ambiental tem incentivado a busca por fontes alternativas de controle desse parasita. Dentro desse contexto, o uso de fitoterápicos aponta como uma alternativa no controle do carrapato, podendo reduzir os impactos econômicos e ambientais ao uso de produtos químicos sintéticos. Assim, o objetivo deste trabalho foi avaliar “in vitro” o efeito dos óleos essenciais de capim-salsar (Cymbopogon interanius Jowitt) e priprioca (Cyperus articulatus L.), substâncias químicas isoladas do timbó (Derris nicou; Derris urucu): rotenona, rotenolona e deguelina e óleos de andiroba (Carapa guianensis Aublet.) e nim (Azadirachta indica A. Juss.), no controle do carrapato. As pesquisas foram desenvolvidas nas instalações da Unidade de Pesquisa Animal “Sen. Álvaro Adolpho”, da Empresa Brasileira de Pesquisa Agropecuária – Embrapa Amazônia Oriental, no município de Belém, estado do Pará, no período de março a julho de 2008. As fêmeas ingurgitadas de Boophilus microplus foram colhidas manualmente, de bovinos naturalmente infestados, em propriedade particular localizada à uma hora de Belém. Após a coleta, foram transportadas para o laboratório em vasilhames plásticos, identificados, limpos e aerados. No laboratório, foram lavadas em água corrente, secas em papel absorvente e pesadas em balança analítica (precisão de 0,0001g). A metodologia utilizada foi a imersão de teleóginas por tempo de cinco minutos. Após a imersão, os carrapatos foram secos em papel absorvente, fixados em placas de Petri e mantidos em temperatura ambiente para realização da postura. Durante todo período experimental a temperatura e umidade relativa do ar em laboratório foi aferida com auxílio de um termohigrometro. O delineamento experimental utilizado foi o inteiramente casualizado. Os percentuais de mortalidade de fêmeas ingurgitadas de B. microplus ,no dia 5 e 15, variaram de: 45% a 90%; 37% a 90% e 60 a 90%; 60% a 90%, para capim-salsar e priprioca, respectivamente. Os resultados referentes a postura não evidenciaram diferença estatística entre as concentrações 2% e 10%, em relação ao controle (água + etanol). Entretanto, houve diferença na concentração 50%, tanto para capim-salsar quanto priprioca. Para capim-salsar, nesta concentração, foi observada postura de ovos inférteis. Com relação aos percentuais de eficiência do produto, os valores obtidos foram de: 100%; 95,63%; 98,77%; 100% e 99,30%; 96,97%; 99,85% e 100%, para os efeitos do óleo essencial de capim-salsar e priprioca nas concentrações 2%, 5%, 10%, 50%, respectivamente. As taxas de mortalidade, no dia 5, variaram de 37% a 41%, 49% a 64% e 52% a 41% para rotenona, rotenolona e deguelina, da menor para maior concentração, respectivamente. Todas as substâncias testadas apresentaram taxa de mortalidade significativamente maior do que as obtidas pelo grupo controle (água + etanol). Os melhores resultados de percentual da postura foram de 49% e 26% para rotenona e deguelina a 200ppm, respectivamente e 34% para rotenolona a 100ppm. Os percentuais de eficiência nos testes com rotenona foram de 95,24% e 98,68%, para rotenolona da ordem de 97,29%; 98,08%; 100% e deguelina 95,45%; 98,23% e 100%, em 100ppm, 200ppm e 300ppm, respectivamente. Para andiroba e nim, o percentual de mortalidade foi observado no dia 15 e demonstrou um comportamento natural do ciclo biológico dos carrapatos, pois não houve diferença estatística significativa entre o controle (água + etanol) e os tratamentos nas diferentes concentrações. Para andiroba e nim a maior eficiência obtida foi de 98,01% e 100%, na concentração de 50%, respectivamente. 2014-02-04T14:14:19Z 2014-02-04T14:14:19Z 2009-02-19 Dissertação DANTAS, Eneida Pontes Mota. Prospecção de biocida em plantas amazônicas e exóticas, visando seu uso racional. 2009. 59 f. Dissertação (Mestrado) - Universidade Federal do Pará, Núcleo de Ciências Agrárias e Desenvolvimento Rural, Empresa Brasileira de Pesquisa Agropecuária, Universidade Federal Rural da Amazônia, Belém, 2009. Programa de Pós-Graduação em Ciência Animal. http://repositorio.ufpa.br/jspui/handle/2011/4713 por Acesso Aberto application/pdf Universidade Federal do Pará Empresa Brasileira de Pesquisa Agropecuária Universidade Federal Rural da Amazônia Brasil Campus Universitário de Castanhal UFPA EMBRAPA UFRA Programa de Pós-Graduação em Ciência Animal |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
por |
| topic |
Ruminantes Bovino Carrapato bovino Boophilus microplus Atividade biocida Controle biológico Praga Óleo aromático Belém - PA Pará - Estado Amazônia brasileira CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| spellingShingle |
Ruminantes Bovino Carrapato bovino Boophilus microplus Atividade biocida Controle biológico Praga Óleo aromático Belém - PA Pará - Estado Amazônia brasileira CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA DANTAS, Eneida Pontes Mota Prospecção de biocida em plantas amazônicas e exóticas, visando seu uso racional |
| topic_facet |
Ruminantes Bovino Carrapato bovino Boophilus microplus Atividade biocida Controle biológico Praga Óleo aromático Belém - PA Pará - Estado Amazônia brasileira CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| description |
The main method of controlling the cattle tick Boophilus microplus is the use of chemical acaricides during the parasite phase. However, the intense use of this method is causing economic
damage and promoting the rapid selection of resistant ticks. Besides the risk of residues in milk,
in meat and environmental contamination. Within this context, the use of herbal points out as an
alternative to control the tick and can reduce the economic and environmental impacts of the use
of synthetic chemicals. On this way, the purpose of this study was to evaluate "in vitro" the effect
of essential oils of grass-salsa (Cymbopogon winteranius Jowitt) and priprioca (Cyperus
articulatus L.), chemicals isolated substances from timbó (Derris nicou; Derris urucu): rotenone,
rotenolone and degueline and Andiroba’s oil (Carapa guianensis Aublet.) and neem (Azadirachta
indica A. Juss.) in the control of ticks. The searches were conducted on the premises of the
Animal Research Unit "Sen. Álvaro Adolpho, of "the Brazilian Enterprise for Agricultural
Research - Embrapa Eastern Amazon, in the city of Belém, state of Pará, in the period from
March to July of 2008. The engorged females of Boophilus microplus were collected manually,
from naturally infected cattle on private property located within an hour of Belém. After
collection, they were transported to the laboratory in plastic containers, marked, cleaned and
aerated. In the laboratory, they were rinsed in running water, dried on absorbent paper and
weighed on analytical balance (accuracy of 0.0001 g). The methodology used was the immersion
of teleogines by time of five minutes. After soaking, the ticks were dried on absorbent paper, set
in petri dishes and kept at room temperature for completion of the posture. Throughout the trial
period the temperature and relative humidity was measured in the laboratory using a
termohigrometer. The experimental design used was completely randomized. The percentage of
mortality of engorged females of B. microplus, on 5 and 15, varied: 45% to 90%, 37% to 90% and
60 to 90%, 60% to 90% for grass-salsa and priprioca respectively. The results for the stance
showed no statistical difference between the concentrations 2% and 10% on the control (water +
ethanol). However, there was a difference in the concentration 50% for both grass-salsa as
priprioca. For grass-salsa, this concentration was observed egg laying infertile. Regarding the
percentage of efficiency of the product, the values obtained were: 100%; 95.63%, 98.77%, 100%
and 99,30%, 96,97%, 99,85% and 100% to the effects of essential oil of salsa and grass-priprioca
concentrations 2%, 5%, 10%, 50%, respectively. Mortality rates, on the 5th , ranged from 37% to
41%, 49% to 64% and 52% to 41% for rotenone, rotenolone and degueline from low to high concentration, respectively. All substances tested showed significantly higher mortality rate than
those obtained by the control group (water + ethanol). The best results were posture of percentage
of 49% and 26% for the rotenone degueline and 200ppm, respectively and 34% for rotenolone at
100ppm. The percentage of efficiency in tests with rotenone were 95.24% and 98.68% for
rotenolone the order of 97.29%, 98.08%, 100% and degueline 95.45%, 98.23% and 100 %, from
100ppm, 200ppm and 300ppm, respectively. To andiroba and neem, the percentage of mortality
was observed on 15 and has a natural behavior of the life cycle of ticks because there was no
statistical difference between the control (water + ethanol) and treatments in different
concentrations. To andiroba and neem the higher efficiency obtained was 98,01% and 100%, at
concentration 50%, respectively. |
| author_additional |
SOUZA FILHO, Antonio Pedro da Silva |
| author_additionalStr |
SOUZA FILHO, Antonio Pedro da Silva |
| format |
Dissertação |
| author |
DANTAS, Eneida Pontes Mota |
| title |
Prospecção de biocida em plantas amazônicas e exóticas, visando seu uso racional |
| title_short |
Prospecção de biocida em plantas amazônicas e exóticas, visando seu uso racional |
| title_full |
Prospecção de biocida em plantas amazônicas e exóticas, visando seu uso racional |
| title_fullStr |
Prospecção de biocida em plantas amazônicas e exóticas, visando seu uso racional |
| title_full_unstemmed |
Prospecção de biocida em plantas amazônicas e exóticas, visando seu uso racional |
| title_sort |
prospecção de biocida em plantas amazônicas e exóticas, visando seu uso racional |
| publisher |
Universidade Federal do Pará |
| publishDate |
2014 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/4713 |
| _version_ |
1787147565555253248 |
| score |
11.653393 |