Therapeutic concentration of morphine reduces oxidative stress in glioma cell line
Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the an...
| Autor principal: | ALMEIDA, Mauro Brito de |
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| Outros Autores: | MALAQUIAS, Allan Costa, NASCIMENTO, José Luiz Martins do, OLIVEIRA, Karen Renata Matos, SILVA, Anderson Manoel Herculano Oliveira da, CRESPO LÓPEZ, Maria Elena |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
2015
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| Acesso em linha: |
http://repositorio.ufpa.br/jspui/handle/2011/6630 |
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ir-2011-6630 |
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ir-2011-66302018-01-10T16:13:59Z Therapeutic concentration of morphine reduces oxidative stress in glioma cell line ALMEIDA, Mauro Brito de MALAQUIAS, Allan Costa NASCIMENTO, José Luiz Martins do OLIVEIRA, Karen Renata Matos SILVA, Anderson Manoel Herculano Oliveira da CRESPO LÓPEZ, Maria Elena Morfina Neuroglia Estresse oxidativo Peróxido de hidrogênio Peroxidação de lipídeos Analgésicos opioides Terapêutica Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and levels of sulfhydryl groups (an indicator of the redox state of the cell). Morphine did not prevent the decrease in cell viability provoked by H2O2) but partially prevented lipid peroxidation caused by 0.0025% H2O2) (a concentration allowing more than 90% cell viability). Interestingly, this opioid did not alter the increased levels of sulfhydryl groups produced by exposure to 0.0025% H2O2), opening the possibility that alternative molecular mechanisms (a direct scavenging activity or the inhibition of NAPDH oxidase) may explain the protective effect registered in the lipid peroxidation assay. Our results demonstrate, for the first time, that morphine in usual analgesic doses may contribute to minimizing oxidative stress in cells of glial origin. This study supports the importance of employing concentrations similar to those used in clinical practice for a better approximation between experimental models and the clinical setting. 2015-05-08T14:09:59Z 2015-05-08T14:09:59Z 2014-05 Artigo de Periódico ALMEIDA, M. B. et al. Therapeutic concentration of morphine reduces oxidative stress in glioma cell line. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 47, n. 5, p. 398-402, maio 2014. Disponível em: <http://www.scielo.br/pdf/bjmbr/v47n5/1414-431X-bjmbr-1414-431X20143697.pdf>. Acesso em: 23 abr. 2015. <http://dx.doi.org/10.1590/1414-431X20143697>. 1414-431X http://repositorio.ufpa.br/jspui/handle/2011/6630 eng Acesso Aberto application/pdf |
| institution |
Repositório Institucional - Universidade Federal do Pará |
| collection |
RI-UFPA |
| language |
eng |
| topic |
Morfina Neuroglia Estresse oxidativo Peróxido de hidrogênio Peroxidação de lipídeos Analgésicos opioides Terapêutica |
| spellingShingle |
Morfina Neuroglia Estresse oxidativo Peróxido de hidrogênio Peroxidação de lipídeos Analgésicos opioides Terapêutica ALMEIDA, Mauro Brito de Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
| topic_facet |
Morfina Neuroglia Estresse oxidativo Peróxido de hidrogênio Peroxidação de lipídeos Analgésicos opioides Terapêutica |
| description |
Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and levels of sulfhydryl groups (an indicator of the redox state of the cell). Morphine did not prevent the decrease in cell viability provoked by H2O2) but partially prevented lipid peroxidation caused by 0.0025% H2O2) (a concentration allowing more than 90% cell viability). Interestingly, this opioid did not alter the increased levels of sulfhydryl groups produced by exposure to 0.0025% H2O2), opening the possibility that alternative molecular mechanisms (a direct scavenging activity or the inhibition of NAPDH oxidase) may explain the protective effect registered in the lipid peroxidation assay. Our results demonstrate, for the first time, that morphine in usual analgesic doses may contribute to minimizing oxidative stress in cells of glial origin. This study supports the importance of employing concentrations similar to those used in clinical practice for a better approximation between experimental models and the clinical setting. |
| format |
Artigo |
| author |
ALMEIDA, Mauro Brito de |
| author2 |
MALAQUIAS, Allan Costa NASCIMENTO, José Luiz Martins do OLIVEIRA, Karen Renata Matos SILVA, Anderson Manoel Herculano Oliveira da CRESPO LÓPEZ, Maria Elena |
| author2Str |
MALAQUIAS, Allan Costa NASCIMENTO, José Luiz Martins do OLIVEIRA, Karen Renata Matos SILVA, Anderson Manoel Herculano Oliveira da CRESPO LÓPEZ, Maria Elena |
| title |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
| title_short |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
| title_full |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
| title_fullStr |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
| title_full_unstemmed |
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
| title_sort |
therapeutic concentration of morphine reduces oxidative stress in glioma cell line |
| publishDate |
2015 |
| url |
http://repositorio.ufpa.br/jspui/handle/2011/6630 |
| _version_ |
1787147757596704768 |
| score |
11.674752 |