Objective: to investigate Leishmania species in a series of autochthonous cutaneous leishmaniasis (CL) cases in Amap?
State, Brazilian Amazon. Methods: this was a descriptive ecological study carried out from January-October/2018 at a reference center for CL diagnosis in Amap?; individuals with CL receiving care from January-May/2018 were recruited; clinical
data and skin biopsies were obtained; from extracted DNA (phenol-chloroform) we amplified the hsp70-234 gene region
(PCR) for nucleotide sequencing (Applied Biosystems: ABI3500XL). Results: 38 individuals were interviewed, examined and
diagnosed; men predominated (28/38; mean age=32.5?11.3); lesions (most ulcers: 37/38) measuring 0,4-10mm (34/38)
and ?11mm (4/38) were multiple in 20/38 individuals; diagnosis of L. braziliensis (1), L. naiffi (1), L. infantum (1), L.
(Viannia) sp. (1), L. amazonensis (2) and L. guyanensis (32); individuals infected with L. guyanensis (32/38) lived in 9/10
municipalities represented in the sample, and 17/32 of these had multiple lesions. Conclusion: presence of Leishmania
guyanensis predominated and was frequently associated with multiple lesions.
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