Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil
Seven isolates from patients with American cutaneous leishmaniasis in the Amazon region of Brazil were phenotypically suggestive of Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids. In this work, two molecular targets were employed to check the hybrid identity of the putative hybrids. Heat sh...
| Autor principal: | Lima, Ana Carolina S |
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| Outros Autores: | Gomes, Claudia Maria C, Tomokane, Thaise Yumie, Campos, Marliane Batista, Zampieri, Ricardo A, Jorge, Carolina L, Laurenti, Marcia D, Silveira, Fernando Tobias, Corbett, Carlos Eduardo P, Floeter-Winter, Lucile Maria |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Sociedade Brasileira de Gen?tica
2021
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4311 |
| Resumo: |
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Seven isolates from patients with American cutaneous leishmaniasis in the Amazon region of Brazil were
phenotypically suggestive of Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids. In this work, two molecular
targets were employed to check the hybrid identity of the putative hybrids. Heat shock protein 70 (hsp70) gene
sequences were analyzed by three different polymerase chain reaction (PCR) approaches, and two different
patterns of inherited hsp70 alleles were found. Three isolates presented heterozygous L. (V.) guyanensis/L. (V.)
shawi patterns, and four presented homozygous hsp70 patterns involving only L. (V.) shawi alleles. The amplicon
sequences confirmed the RFLP patterns. The high-resolution melting method detected variant heterozygous and
homozygous profiles. Single-nucleotide polymorphism genotyping/cleaved amplified polymorphic site analysis
suggested a higher contribution from L. (V.) guyanensis in hsp70 heterozygous hybrids. Additionally, PCR-RFLP
analysis targeting the enzyme mannose phosphate isomerase (mpi) gene indicated heterozygous and homozygous
cleavage patterns for L. (V.) shawi and L. (V.) guyanensis, corroborating the hsp70 findings. In this communication,
we present molecular findings based on partial informative regions of the coding sequences of hsp70 and mpi as
markers confirming that some of the parasite strains from the Brazilian Amazon region are indeed hybrids between
L. (V.) guyanensis and L. (V.) shawi. |