Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amap?, Brazil, 2018
Objective: to investigate Leishmania species in a series of autochthonous cutaneous leishmaniasis (CL) cases in Amap? State, Brazilian Amazon. Methods: this was a descriptive ecological study carried out from January-October/2018 at a reference center for CL diagnosis in Amap?; individuals with CL...
| Autor principal: | Almeida, Ariely Nunes Ferreira de |
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| Outros Autores: | Nascimento, Luciana de C?ssia Silva do, Sousa, Edith Silvia Moura de Moura, Oliveira, Afonso Jos? Diger de, Sena, Maria Gorete de, Resende, Breno Mau?s de, Chaves, Raimunda Cleide Gon?alves, Santos, Lourdes Maria Garcez dos |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de
2020
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| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4082 |
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ir-iec-4082 |
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ir-iec-40822020-04-07T16:53:08Z Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amap?, Brazil, 2018 Vigil?ncia da leishmaniose cut?nea em amostras cl?nicas: distribui??o da Leishmania guyanensis no estado do Amap?, 2018 Almeida, Ariely Nunes Ferreira de Nascimento, Luciana de C?ssia Silva do Sousa, Edith Silvia Moura de Moura Oliveira, Afonso Jos? Diger de Sena, Maria Gorete de Resende, Breno Mau?s de Chaves, Raimunda Cleide Gon?alves Santos, Lourdes Maria Garcez dos Leishmaniose Cut?nea / patologia Leishmania guyanensis / parasitologia Epidemiologia Molecular / m?todos Ecossistema Amaz?nico Amap? (AP) Objective: to investigate Leishmania species in a series of autochthonous cutaneous leishmaniasis (CL) cases in Amap? State, Brazilian Amazon. Methods: this was a descriptive ecological study carried out from January-October/2018 at a reference center for CL diagnosis in Amap?; individuals with CL receiving care from January-May/2018 were recruited; clinical data and skin biopsies were obtained; from extracted DNA (phenol-chloroform) we amplified the hsp70-234 gene region (PCR) for nucleotide sequencing (Applied Biosystems: ABI3500XL). Results: 38 individuals were interviewed, examined and diagnosed; men predominated (28/38; mean age=32.5?11.3); lesions (most ulcers: 37/38) measuring 0,4-10mm (34/38) and ?11mm (4/38) were multiple in 20/38 individuals; diagnosis of L. braziliensis (1), L. naiffi (1), L. infantum (1), L. (Viannia) sp. (1), L. amazonensis (2) and L. guyanensis (32); individuals infected with L. guyanensis (32/38) lived in 9/10 municipalities represented in the sample, and 17/32 of these had multiple lesions. Conclusion: presence of Leishmania guyanensis predominated and was frequently associated with multiple lesions. This research received financial support from the Amap? State Research Support Foundation (FAPEAP) ? Process No. 250.203.039/2016 ? and a doctoral Research Grant from the Par? State Research Support Foundation (FAPESPA) ? Process No. 003/2014. 2020-04-07T16:41:45Z 2020-04-07T16:41:45Z 2020 Artigo ALMEIDA, Ariely Nunes Ferreira de et al. Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amap?, Brazil, 2018. Epidemiologia e Servi?os de Sa?de, v. 29, n. 1, e2018504, 2020. 2237-9622 http://patua.iec.gov.br//handle/iec/4082 10.5123/S1679-49742020000100007 eng Acesso Aberto application/pdf Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Leishmaniose Cut?nea / patologia Leishmania guyanensis / parasitologia Epidemiologia Molecular / m?todos Ecossistema Amaz?nico Amap? (AP) |
| spellingShingle |
Leishmaniose Cut?nea / patologia Leishmania guyanensis / parasitologia Epidemiologia Molecular / m?todos Ecossistema Amaz?nico Amap? (AP) Almeida, Ariely Nunes Ferreira de Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amap?, Brazil, 2018 |
| topic_facet |
Leishmaniose Cut?nea / patologia Leishmania guyanensis / parasitologia Epidemiologia Molecular / m?todos Ecossistema Amaz?nico Amap? (AP) |
| description |
Objective: to investigate Leishmania species in a series of autochthonous cutaneous leishmaniasis (CL) cases in Amap?
State, Brazilian Amazon. Methods: this was a descriptive ecological study carried out from January-October/2018 at a reference center for CL diagnosis in Amap?; individuals with CL receiving care from January-May/2018 were recruited; clinical
data and skin biopsies were obtained; from extracted DNA (phenol-chloroform) we amplified the hsp70-234 gene region
(PCR) for nucleotide sequencing (Applied Biosystems: ABI3500XL). Results: 38 individuals were interviewed, examined and
diagnosed; men predominated (28/38; mean age=32.5?11.3); lesions (most ulcers: 37/38) measuring 0,4-10mm (34/38)
and ?11mm (4/38) were multiple in 20/38 individuals; diagnosis of L. braziliensis (1), L. naiffi (1), L. infantum (1), L.
(Viannia) sp. (1), L. amazonensis (2) and L. guyanensis (32); individuals infected with L. guyanensis (32/38) lived in 9/10
municipalities represented in the sample, and 17/32 of these had multiple lesions. Conclusion: presence of Leishmania
guyanensis predominated and was frequently associated with multiple lesions. |
| format |
Artigo |
| author |
Almeida, Ariely Nunes Ferreira de |
| author2 |
Nascimento, Luciana de C?ssia Silva do Sousa, Edith Silvia Moura de Moura Oliveira, Afonso Jos? Diger de Sena, Maria Gorete de Resende, Breno Mau?s de Chaves, Raimunda Cleide Gon?alves Santos, Lourdes Maria Garcez dos |
| author2Str |
Nascimento, Luciana de C?ssia Silva do Sousa, Edith Silvia Moura de Moura Oliveira, Afonso Jos? Diger de Sena, Maria Gorete de Resende, Breno Mau?s de Chaves, Raimunda Cleide Gon?alves Santos, Lourdes Maria Garcez dos |
| title |
Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amap?, Brazil, 2018 |
| title_short |
Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amap?, Brazil, 2018 |
| title_full |
Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amap?, Brazil, 2018 |
| title_fullStr |
Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amap?, Brazil, 2018 |
| title_full_unstemmed |
Surveillance of cutaneous leishmaniasis in clinical samples: distribution of Leishmania guyanensis in the state of Amap?, Brazil, 2018 |
| title_sort |
surveillance of cutaneous leishmaniasis in clinical samples: distribution of leishmania guyanensis in the state of amap?, brazil, 2018 |
| publisher |
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de |
| publishDate |
2020 |
| url |
http://patua.iec.gov.br//handle/iec/4082 |
| _version_ |
1717584428301025280 |
| score |
11.653393 |