Detection of drug resistant Mycobacterium Tuberculosis strains using kit SIRE Nitratase?: a multicenter study
Background: The Commercial Kit SIRE Nitratase? PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy,...
| Autor principal: | Miranda, Silvana Spindola de |
|---|---|
| Outros Autores: | Almeida, Isabela Neves de, Mansur, Maria de F?tima Filardi Oliveira, Figueredo, Lida Jouca de Assis, Carvalho, W?nia da Silva, Hadaad, Jo?o Paulo Amaral, Diniz, Jaciara de Lourdes do Carmo Guimar?es, Groll, Andrea Von, Silva, Pedro Almeida da, Lopes, Maria Luiza, Santos, Marcelo Cordeiro dos, Brito, Alexandra, Mello, Fernanda Carvalho de Queiroz, Malaquias, Thiago da Silva Santos, Croda, Julio, Pinhata, Juliana Maira Watanabe, Oliveira, Ros?ngela Siqueira de, Chimara, Erica, Rossetti, Maria L?cia, Halon, Maria Laura, Louren?o, Maria Cristina, Medeiros, Reginalda Ferreira de Melo, Montes, F?tima Cristina Onofre Fandinho, Machado, Diana, Vivieiros, Miguel, Kritski, Afr?nio Lineu |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Instituto de Tecnologia do Paran?
2020
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| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4147 |
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ir-iec-4147 |
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ir-iec-41472020-08-17T17:11:32Z Detection of drug resistant Mycobacterium Tuberculosis strains using kit SIRE Nitratase?: a multicenter study Miranda, Silvana Spindola de Almeida, Isabela Neves de Mansur, Maria de F?tima Filardi Oliveira Figueredo, Lida Jouca de Assis Carvalho, W?nia da Silva Hadaad, Jo?o Paulo Amaral Diniz, Jaciara de Lourdes do Carmo Guimar?es Groll, Andrea Von Silva, Pedro Almeida da Lopes, Maria Luiza Santos, Marcelo Cordeiro dos Brito, Alexandra Mello, Fernanda Carvalho de Queiroz Malaquias, Thiago da Silva Santos Croda, Julio Pinhata, Juliana Maira Watanabe Oliveira, Ros?ngela Siqueira de Chimara, Erica Rossetti, Maria L?cia Halon, Maria Laura Louren?o, Maria Cristina Medeiros, Reginalda Ferreira de Melo Montes, F?tima Cristina Onofre Fandinho Machado, Diana Vivieiros, Miguel Kritski, Afr?nio Lineu Tuberculose / diagn?stico Resist?ncia a M?ltiplos Medicamentos Mycobacterium / efeitos dos f?rmacos Sensibilidade e Especificidade Nitrato Redutase / farmacologia Estudo Multic?ntrico Background: The Commercial Kit SIRE Nitratase? PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries. This research was funded by MINAS GERAIS STATE RESEARCH SUPPORT FOUNDATION (FAPEMIG), grants numbers 65/10 and CDS-APQ-03266-13, and by NATIONAL COUNCIL FOR SCIENTIFIC AND TECHNOLOGICAL DEVELOPMENT (CNPQ) grants numbers 310174/2014-7 and 446796/2014-0. 2020-08-17T16:37:15Z 2020-08-17T16:37:15Z 2020 Artigo MIRANDA, Silvana Spindola de et al. Detection of drug resistant Mycobacterium Tuberculosis strains using kit SIRE Nitratase?: a multicenter study. Brazilian Archives of Biology and Technology, v. 63, e20190179, 2020. 1678-4324 http://patua.iec.gov.br//handle/iec/4147 10.1590/1678-4324-2020190179 eng Acesso Aberto application/pdf Instituto de Tecnologia do Paran? |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Tuberculose / diagn?stico Resist?ncia a M?ltiplos Medicamentos Mycobacterium / efeitos dos f?rmacos Sensibilidade e Especificidade Nitrato Redutase / farmacologia Estudo Multic?ntrico |
| spellingShingle |
Tuberculose / diagn?stico Resist?ncia a M?ltiplos Medicamentos Mycobacterium / efeitos dos f?rmacos Sensibilidade e Especificidade Nitrato Redutase / farmacologia Estudo Multic?ntrico Miranda, Silvana Spindola de Detection of drug resistant Mycobacterium Tuberculosis strains using kit SIRE Nitratase?: a multicenter study |
| topic_facet |
Tuberculose / diagn?stico Resist?ncia a M?ltiplos Medicamentos Mycobacterium / efeitos dos f?rmacos Sensibilidade e Especificidade Nitrato Redutase / farmacologia Estudo Multic?ntrico |
| description |
Background: The Commercial Kit SIRE Nitratase? PlastLabor, is a drug susceptibility test kit
used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study
aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the
proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold
standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis
clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960
system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and
98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can
rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not
require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income
countries. |
| format |
Artigo |
| author |
Miranda, Silvana Spindola de |
| author2 |
Almeida, Isabela Neves de Mansur, Maria de F?tima Filardi Oliveira Figueredo, Lida Jouca de Assis Carvalho, W?nia da Silva Hadaad, Jo?o Paulo Amaral Diniz, Jaciara de Lourdes do Carmo Guimar?es Groll, Andrea Von Silva, Pedro Almeida da Lopes, Maria Luiza Santos, Marcelo Cordeiro dos Brito, Alexandra Mello, Fernanda Carvalho de Queiroz Malaquias, Thiago da Silva Santos Croda, Julio Pinhata, Juliana Maira Watanabe Oliveira, Ros?ngela Siqueira de Chimara, Erica Rossetti, Maria L?cia Halon, Maria Laura Louren?o, Maria Cristina Medeiros, Reginalda Ferreira de Melo Montes, F?tima Cristina Onofre Fandinho Machado, Diana Vivieiros, Miguel Kritski, Afr?nio Lineu |
| author2Str |
Almeida, Isabela Neves de Mansur, Maria de F?tima Filardi Oliveira Figueredo, Lida Jouca de Assis Carvalho, W?nia da Silva Hadaad, Jo?o Paulo Amaral Diniz, Jaciara de Lourdes do Carmo Guimar?es Groll, Andrea Von Silva, Pedro Almeida da Lopes, Maria Luiza Santos, Marcelo Cordeiro dos Brito, Alexandra Mello, Fernanda Carvalho de Queiroz Malaquias, Thiago da Silva Santos Croda, Julio Pinhata, Juliana Maira Watanabe Oliveira, Ros?ngela Siqueira de Chimara, Erica Rossetti, Maria L?cia Halon, Maria Laura Louren?o, Maria Cristina Medeiros, Reginalda Ferreira de Melo Montes, F?tima Cristina Onofre Fandinho Machado, Diana Vivieiros, Miguel Kritski, Afr?nio Lineu |
| title |
Detection of drug resistant Mycobacterium Tuberculosis strains using kit SIRE Nitratase?: a multicenter study |
| title_short |
Detection of drug resistant Mycobacterium Tuberculosis strains using kit SIRE Nitratase?: a multicenter study |
| title_full |
Detection of drug resistant Mycobacterium Tuberculosis strains using kit SIRE Nitratase?: a multicenter study |
| title_fullStr |
Detection of drug resistant Mycobacterium Tuberculosis strains using kit SIRE Nitratase?: a multicenter study |
| title_full_unstemmed |
Detection of drug resistant Mycobacterium Tuberculosis strains using kit SIRE Nitratase?: a multicenter study |
| title_sort |
detection of drug resistant mycobacterium tuberculosis strains using kit sire nitratase?: a multicenter study |
| publisher |
Instituto de Tecnologia do Paran? |
| publishDate |
2020 |
| url |
http://patua.iec.gov.br//handle/iec/4147 |
| _version_ |
1717584381147611136 |
| score |
11.674684 |