Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil
Seven isolates from patients with American cutaneous leishmaniasis in the Amazon region of Brazil were phenotypically suggestive of Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids. In this work, two molecular targets were employed to check the hybrid identity of the putative hybrids. Heat sh...
| Autor principal: | Lima, Ana Carolina S |
|---|---|
| Outros Autores: | Gomes, Claudia Maria C, Tomokane, Thaise Yumie, Campos, Marliane Batista, Zampieri, Ricardo A, Jorge, Carolina L, Laurenti, Marcia D, Silveira, Fernando Tobias, Corbett, Carlos Eduardo P, Floeter-Winter, Lucile Maria |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Sociedade Brasileira de Gen?tica
2021
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4311 |
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ir-iec-4311 |
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ir-iec-43112021-05-24T17:10:48Z Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil Lima, Ana Carolina S Gomes, Claudia Maria C Tomokane, Thaise Yumie Campos, Marliane Batista Zampieri, Ricardo A Jorge, Carolina L Laurenti, Marcia D Silveira, Fernando Tobias Corbett, Carlos Eduardo P Floeter-Winter, Lucile Maria Leishmaniose Cut?nea / patologia Rea??o em Cadeia da Polimerase / m?todos Ecossistema Amaz?nico Seven isolates from patients with American cutaneous leishmaniasis in the Amazon region of Brazil were phenotypically suggestive of Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids. In this work, two molecular targets were employed to check the hybrid identity of the putative hybrids. Heat shock protein 70 (hsp70) gene sequences were analyzed by three different polymerase chain reaction (PCR) approaches, and two different patterns of inherited hsp70 alleles were found. Three isolates presented heterozygous L. (V.) guyanensis/L. (V.) shawi patterns, and four presented homozygous hsp70 patterns involving only L. (V.) shawi alleles. The amplicon sequences confirmed the RFLP patterns. The high-resolution melting method detected variant heterozygous and homozygous profiles. Single-nucleotide polymorphism genotyping/cleaved amplified polymorphic site analysis suggested a higher contribution from L. (V.) guyanensis in hsp70 heterozygous hybrids. Additionally, PCR-RFLP analysis targeting the enzyme mannose phosphate isomerase (mpi) gene indicated heterozygous and homozygous cleavage patterns for L. (V.) shawi and L. (V.) guyanensis, corroborating the hsp70 findings. In this communication, we present molecular findings based on partial informative regions of the coding sequences of hsp70 and mpi as markers confirming that some of the parasite strains from the Brazilian Amazon region are indeed hybrids between L. (V.) guyanensis and L. (V.) shawi. FAPESP (2012/08338-8; 14/50315-0). 2021-05-24T16:54:00Z 2021-05-24T16:54:00Z 2021 Artigo LIMA, Ana Carolina S. et al. Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil. Genetics and Molecular Biology, v. 44, n. 2, p. 1-8, 2021. 1678-4685 http://patua.iec.gov.br//handle/iec/4311 10.1590/1678-4685-GMB-2020-0123 eng Acesso Aberto application/pdf Sociedade Brasileira de Gen?tica |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Leishmaniose Cut?nea / patologia Rea??o em Cadeia da Polimerase / m?todos Ecossistema Amaz?nico |
| spellingShingle |
Leishmaniose Cut?nea / patologia Rea??o em Cadeia da Polimerase / m?todos Ecossistema Amaz?nico Lima, Ana Carolina S Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
| topic_facet |
Leishmaniose Cut?nea / patologia Rea??o em Cadeia da Polimerase / m?todos Ecossistema Amaz?nico |
| description |
Seven isolates from patients with American cutaneous leishmaniasis in the Amazon region of Brazil were
phenotypically suggestive of Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids. In this work, two molecular
targets were employed to check the hybrid identity of the putative hybrids. Heat shock protein 70 (hsp70) gene
sequences were analyzed by three different polymerase chain reaction (PCR) approaches, and two different
patterns of inherited hsp70 alleles were found. Three isolates presented heterozygous L. (V.) guyanensis/L. (V.)
shawi patterns, and four presented homozygous hsp70 patterns involving only L. (V.) shawi alleles. The amplicon
sequences confirmed the RFLP patterns. The high-resolution melting method detected variant heterozygous and
homozygous profiles. Single-nucleotide polymorphism genotyping/cleaved amplified polymorphic site analysis
suggested a higher contribution from L. (V.) guyanensis in hsp70 heterozygous hybrids. Additionally, PCR-RFLP
analysis targeting the enzyme mannose phosphate isomerase (mpi) gene indicated heterozygous and homozygous
cleavage patterns for L. (V.) shawi and L. (V.) guyanensis, corroborating the hsp70 findings. In this communication,
we present molecular findings based on partial informative regions of the coding sequences of hsp70 and mpi as
markers confirming that some of the parasite strains from the Brazilian Amazon region are indeed hybrids between
L. (V.) guyanensis and L. (V.) shawi. |
| format |
Artigo |
| author |
Lima, Ana Carolina S |
| author2 |
Gomes, Claudia Maria C Tomokane, Thaise Yumie Campos, Marliane Batista Zampieri, Ricardo A Jorge, Carolina L Laurenti, Marcia D Silveira, Fernando Tobias Corbett, Carlos Eduardo P Floeter-Winter, Lucile Maria |
| author2Str |
Gomes, Claudia Maria C Tomokane, Thaise Yumie Campos, Marliane Batista Zampieri, Ricardo A Jorge, Carolina L Laurenti, Marcia D Silveira, Fernando Tobias Corbett, Carlos Eduardo P Floeter-Winter, Lucile Maria |
| title |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
| title_short |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
| title_full |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
| title_fullStr |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
| title_full_unstemmed |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
| title_sort |
molecular tools confirm natural leishmania (viannia) guyanensis/l. (v.) shawi hybrids causing cutaneous leishmaniasis in the amazon region of brazil |
| publisher |
Sociedade Brasileira de Gen?tica |
| publishDate |
2021 |
| url |
http://patua.iec.gov.br//handle/iec/4311 |
| _version_ |
1717584382492934144 |
| score |
11.653393 |