Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil
Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmania-specific monoclonal antibodies (MAbs), and by PCR...
| Autor principal: | Grisard, Edmundo C |
|---|---|
| Outros Autores: | Steindel, M?rio, Shaw, Jeffrey Jon, Ishikawa, Edna A. Y, Carvalho-Pinto, Carlos J, Eger-Mangrich, Iriane, Toma, Helena K, Lima, Jos? H, Romanha, Alvaro J, Campbell, David A |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Elsevier
2021
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4354 |
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ir-iec-4354 |
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ir-iec-43542021-07-15T14:31:10Z Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil Grisard, Edmundo C Steindel, M?rio Shaw, Jeffrey Jon Ishikawa, Edna A. Y Carvalho-Pinto, Carlos J Eger-Mangrich, Iriane Toma, Helena K Lima, Jos? H Romanha, Alvaro J Campbell, David A Leishmaniose Cut?nea / parasitologia Anticorpos Monoclonais Hibridiza??o In Situ / m?todos Rea??o em Cadeia da Polimerase / m?todos Transmiss?o de Doen?a Infecciosa Santa Catarina (SC) Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmania-specific monoclonal antibodies (MAbs), and by PCR amplification and hybridization assay of the mini-exon gene with group specific probes. The results obtained with the MAbs were in agreement with the genetic marker. Two isolates (MHOM/BR/89/JSC89-H1 and MHOM/BR/89/JSC89-H2) were identified as L. (Leishmania) amazonensis and two (MHOM/BR/96/LSC96-H3 and MHOM/BR/97/LSC97-H4) as L. (Viannia) braziliensis. The southernmost autochthonous cases of ACL in Brazil are due to two different Leishmania sp. species, confirming the spreading of ACL on the American continent. 2021-07-15T14:11:42Z 2021-07-15T14:11:42Z 2000 Artigo GRISARD, Edmundo C. et al. Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil. Acta Tropica, v. 74, n. 1, p. 89-93, Jan. 2000. 0001-706X http://patua.iec.gov.br//handle/iec/4354 10.1016/S0001-706X(99)00054-6 eng Acesso Aberto application/pdf Elsevier |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Leishmaniose Cut?nea / parasitologia Anticorpos Monoclonais Hibridiza??o In Situ / m?todos Rea??o em Cadeia da Polimerase / m?todos Transmiss?o de Doen?a Infecciosa Santa Catarina (SC) |
| spellingShingle |
Leishmaniose Cut?nea / parasitologia Anticorpos Monoclonais Hibridiza??o In Situ / m?todos Rea??o em Cadeia da Polimerase / m?todos Transmiss?o de Doen?a Infecciosa Santa Catarina (SC) Grisard, Edmundo C Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil |
| topic_facet |
Leishmaniose Cut?nea / parasitologia Anticorpos Monoclonais Hibridiza??o In Situ / m?todos Rea??o em Cadeia da Polimerase / m?todos Transmiss?o de Doen?a Infecciosa Santa Catarina (SC) |
| description |
Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmania-specific monoclonal antibodies (MAbs), and by PCR amplification and hybridization assay of the mini-exon gene with group specific probes. The results obtained with the MAbs were in agreement with the genetic marker. Two isolates (MHOM/BR/89/JSC89-H1 and MHOM/BR/89/JSC89-H2) were identified as L. (Leishmania) amazonensis and two (MHOM/BR/96/LSC96-H3 and MHOM/BR/97/LSC97-H4) as L. (Viannia) braziliensis. The southernmost autochthonous cases of ACL in Brazil are due to two different Leishmania sp. species, confirming the spreading of ACL on the American continent. |
| format |
Artigo |
| author |
Grisard, Edmundo C |
| author2 |
Steindel, M?rio Shaw, Jeffrey Jon Ishikawa, Edna A. Y Carvalho-Pinto, Carlos J Eger-Mangrich, Iriane Toma, Helena K Lima, Jos? H Romanha, Alvaro J Campbell, David A |
| author2Str |
Steindel, M?rio Shaw, Jeffrey Jon Ishikawa, Edna A. Y Carvalho-Pinto, Carlos J Eger-Mangrich, Iriane Toma, Helena K Lima, Jos? H Romanha, Alvaro J Campbell, David A |
| title |
Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil |
| title_short |
Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil |
| title_full |
Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil |
| title_fullStr |
Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil |
| title_full_unstemmed |
Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil |
| title_sort |
characterization of leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in santa catarina state, southern brazil |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
http://patua.iec.gov.br//handle/iec/4354 |
| _version_ |
1717584486220169216 |
| score |
11.675608 |