Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae
Premise of the study: Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. Methods...
| Autor principal: | Ramos, Santiago Linorio Ferreyra |
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| Outros Autores: | Macedo, Jeferson Luis Vasconcelos de, Lopes, Maria Teresa Gomes, Batista, Jacqueline da Silva, Formiga, Kyara M., Silva, Perla Pimentel da, Saulo-Machado, Antonio C., Veasey, Elizabeth Ann |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
American Journal of Botany
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17966 |
| Resumo: |
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Premise of the study: Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. Methods and Results: Fourteen microsatellite loci were isolated from a microsatellite-enriched genomic library and used to characterize two wild populations of tucumã of Amazonas (Manaus and Manicoré cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae. Conclusions: The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucumã. © 2012 Botanical Society of America. |