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Artigo
Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae
Premise of the study: Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. Methods...
Autor principal: | Ramos, Santiago Linorio Ferreyra |
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Outros Autores: | Macedo, Jeferson Luis Vasconcelos de, Lopes, Maria Teresa Gomes, Batista, Jacqueline da Silva, Formiga, Kyara M., Silva, Perla Pimentel da, Saulo-Machado, Antonio C., Veasey, Elizabeth Ann |
Grau: | Artigo |
Idioma: | English |
Publicado em: |
American Journal of Botany
2020
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https://repositorio.inpa.gov.br/handle/1/17966 |
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oai:repositorio:1-17966 Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae Ramos, Santiago Linorio Ferreyra Macedo, Jeferson Luis Vasconcelos de Lopes, Maria Teresa Gomes Batista, Jacqueline da Silva Formiga, Kyara M. Silva, Perla Pimentel da Saulo-Machado, Antonio C. Veasey, Elizabeth Ann Microsatellite Dna Dna, Plant Primer Dna Allele Genetic Marker Genetic Variation Heterozygosity Molecular Analysis Monocotyledon Polymorphism Population Structure Reproductive Strategy Species Richness Wild Population Arecaceae Brasil Dna Sequence Polymorphism, Genetic Genetics Molecular Genetics Nucleotide Sequence Polymerase Chain Reaction Arecaceae Brasil Dna Primers Dna, Plant Microsatellite Repeats Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Sequence Analysis, Dna Amazonas Amazonia Brasil Manaus Premise of the study: Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. Methods and Results: Fourteen microsatellite loci were isolated from a microsatellite-enriched genomic library and used to characterize two wild populations of tucumã of Amazonas (Manaus and Manicoré cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae. Conclusions: The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucumã. © 2012 Botanical Society of America. 2020-06-15T21:50:32Z 2020-06-15T21:50:32Z 2012 Artigo https://repositorio.inpa.gov.br/handle/1/17966 10.3732/ajb.1100607 en Volume 99, Número 12, Pags. e508-e510 Restrito American Journal of Botany |
institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
collection |
INPA-RI |
language |
English |
topic |
Microsatellite Dna Dna, Plant Primer Dna Allele Genetic Marker Genetic Variation Heterozygosity Molecular Analysis Monocotyledon Polymorphism Population Structure Reproductive Strategy Species Richness Wild Population Arecaceae Brasil Dna Sequence Polymorphism, Genetic Genetics Molecular Genetics Nucleotide Sequence Polymerase Chain Reaction Arecaceae Brasil Dna Primers Dna, Plant Microsatellite Repeats Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Sequence Analysis, Dna Amazonas Amazonia Brasil Manaus |
spellingShingle |
Microsatellite Dna Dna, Plant Primer Dna Allele Genetic Marker Genetic Variation Heterozygosity Molecular Analysis Monocotyledon Polymorphism Population Structure Reproductive Strategy Species Richness Wild Population Arecaceae Brasil Dna Sequence Polymorphism, Genetic Genetics Molecular Genetics Nucleotide Sequence Polymerase Chain Reaction Arecaceae Brasil Dna Primers Dna, Plant Microsatellite Repeats Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Sequence Analysis, Dna Amazonas Amazonia Brasil Manaus Ramos, Santiago Linorio Ferreyra Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae |
topic_facet |
Microsatellite Dna Dna, Plant Primer Dna Allele Genetic Marker Genetic Variation Heterozygosity Molecular Analysis Monocotyledon Polymorphism Population Structure Reproductive Strategy Species Richness Wild Population Arecaceae Brasil Dna Sequence Polymorphism, Genetic Genetics Molecular Genetics Nucleotide Sequence Polymerase Chain Reaction Arecaceae Brasil Dna Primers Dna, Plant Microsatellite Repeats Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Sequence Analysis, Dna Amazonas Amazonia Brasil Manaus |
description |
Premise of the study: Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. Methods and Results: Fourteen microsatellite loci were isolated from a microsatellite-enriched genomic library and used to characterize two wild populations of tucumã of Amazonas (Manaus and Manicoré cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae. Conclusions: The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucumã. © 2012 Botanical Society of America. |
format |
Artigo |
author |
Ramos, Santiago Linorio Ferreyra |
author2 |
Macedo, Jeferson Luis Vasconcelos de Lopes, Maria Teresa Gomes Batista, Jacqueline da Silva Formiga, Kyara M. Silva, Perla Pimentel da Saulo-Machado, Antonio C. Veasey, Elizabeth Ann |
author2Str |
Macedo, Jeferson Luis Vasconcelos de Lopes, Maria Teresa Gomes Batista, Jacqueline da Silva Formiga, Kyara M. Silva, Perla Pimentel da Saulo-Machado, Antonio C. Veasey, Elizabeth Ann |
title |
Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae |
title_short |
Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae |
title_full |
Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae |
title_fullStr |
Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae |
title_full_unstemmed |
Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae |
title_sort |
microsatellite loci for tucumã of amazonas (astrocaryum aculeatum) and amplification in other arecaceae |
publisher |
American Journal of Botany |
publishDate |
2020 |
url |
https://repositorio.inpa.gov.br/handle/1/17966 |
_version_ |
1787141490493882368 |
score |
11.653393 |