Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated a...
| Autor principal: | Guimarães-Marques, Giselle Moura |
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| Outros Autores: | Batista, Jacqueline da Silva, Guimarães, H. M., Naice-Daou, M. V., Lima, M. P., Formiga, Kyara M., Santos, Joselita Maria M., Lima, A. C., Rafael, Míriam Silva |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Research
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14997 |
| Resumo: |
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The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species. |