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Dissertação
Isolamento, caracterização e atividade antimicrobiana de metabólitos secundários produzidos por Streptomyces sp. de solo amazônico
The Amazon Rainforest is known as one of the main vectors rich in plants and animals with wide genetic range. However, there is little reference to the microbial of this biome. Therefore, it is important to investigate an autochthonous microbiodiversity and describe its biotechnological potent...
Autor principal: | LIBERAL, Talita Cunha de Faria |
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Grau: | Dissertação |
Idioma: | pt_BR |
Publicado em: |
Universidade Federal do Oeste do Pará
2021
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Assuntos: | |
Acesso em linha: |
https://repositorio.ufopa.edu.br/jspui/handle/123456789/482 |
Resumo: |
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The Amazon Rainforest is known as one of the main vectors rich in plants and animals
with wide genetic range. However, there is little reference to the microbial of this biome.
Therefore, it is important to investigate an autochthonous microbiodiversity and describe its
biotechnological potential. The genus Streptomyces is the important major biotechnology
within the group of actinobacteria, being leading to the production of large part of antibiotics
has known and by various various enzymes of industrial applications. This work aimed at the
production of secondary antimicrobial metabolites and enzymes of industrial interest, by
Streptomyces sp. soil of rhizospheric soil of a plant native to the Amazon. The actinobacteria
line isolated from rhizospheric soil of Aniba parviflora Syn Fragans (Macacaporanga) was
named MPO11, after identification by classical and molecular methods, presenting 99.9%
similarity with Streptomyces cinereus. Regarding its antimicrobial potential, an activity of
microorganisms of clinical and phytopathogenic interest was evaluated. The subproduction of
MPO11 submersion, a type of action with antibacterial and phytopathogenic action, besides
the production of several extracellular enzymes of industrial interest such as caseinase, lipase
and catalase. The mean physiological and biochemical profile was salinity tolerant at
concentrations of 3 to 5% NaCl. The ethyl acetate extract obtained from the submerged was
subjected to reverse phase High Performance Liquid Chromatography to optimize the
isolation of the metabolites present in the extract, which according to mass spectrometry. The
chemical analysis of the three data sources undergoing structural elucidation and suggests that
the class of macrolides, commonly found in the genus Streptomyces. Thus, the present work
can contribute to the study of the microbiodiversity of this biome and the discovery of its
biotechnological potential in the production of bioactive substances of microbial origin. |