Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages ? C6/36, VERO, BHK-21, and LLC-MK2 ? under the multiplici...
| Autor principal: | Ribeiro, Ana Cl?udia da Silva |
|---|---|
| Outros Autores: | Carvalho, Carlos Alberto Marques de, Casseb, Samir Mansour Moraes, Rodrigues, Sueli Guerreiro, Vasconcelos, Pedro Fernando da Costa, Carvalho, Val?ria Lima |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
MS/SVS/Instituto Evandro Chagas
2019
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| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/3883 |
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ir-iec-3883 |
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ir-iec-38832019-09-04T18:13:15Z Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages Perfis de infec??o do v?rus Mayaro e do v?rus Chikungunya em linhagens de c?lulas de mam?feros e mosquitos Ribeiro, Ana Cl?udia da Silva Carvalho, Carlos Alberto Marques de Casseb, Samir Mansour Moraes Rodrigues, Sueli Guerreiro Vasconcelos, Pedro Fernando da Costa Carvalho, Val?ria Lima Arbovirus / isolamento & purifica??o Alphavirus / isolamento & purifica??o V?rus Chikungunya / isolamento & purifica??o V?rus Mayaro Linhagem Celular / citologia OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages ? C6/36, VERO, BHK-21, and LLC-MK2 ? under the multiplicity of infection of 1 PFU/cell, which were monitored for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21 cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays, all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2 cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their diagnosis in biological samples. This work was supported by grants from Minist?rio da Sa?de, Brazil, Instituto Nacional de Ci?ncia e Tecnologia de Febres Hemorr?gicas Virais (grant no. 573739/2008-0), and Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (grant no. 457664/2013-4). 2019-09-04T17:56:14Z 2019-09-04T17:56:14Z 2018 Artigo RIBEIRO, Ana Cl?udia da Silva et al. Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages. Revista Pan-Amaz?nica de Sa?de, v. 9, n. 4, p. 25-35, dez. 2018. Disponivel em http://scielo.iec.gov.br/pdf/rpas/v9n4/2176-6223-rpas-9-04-25.pdf. Acesso em 04 set. 2019. 2176-6223 http://patua.iec.gov.br//handle/iec/3883 10.5123/s2176-62232018000400003 eng Acesso Aberto application/pdf MS/SVS/Instituto Evandro Chagas |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Arbovirus / isolamento & purifica??o Alphavirus / isolamento & purifica??o V?rus Chikungunya / isolamento & purifica??o V?rus Mayaro Linhagem Celular / citologia |
| spellingShingle |
Arbovirus / isolamento & purifica??o Alphavirus / isolamento & purifica??o V?rus Chikungunya / isolamento & purifica??o V?rus Mayaro Linhagem Celular / citologia Ribeiro, Ana Cl?udia da Silva Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
| topic_facet |
Arbovirus / isolamento & purifica??o Alphavirus / isolamento & purifica??o V?rus Chikungunya / isolamento & purifica??o V?rus Mayaro Linhagem Celular / citologia |
| description |
OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell
lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell
lineages ? C6/36, VERO, BHK-21, and LLC-MK2 ? under the multiplicity of infection of 1 PFU/cell, which were monitored
for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were
subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR)
assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21
cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays,
all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR
assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2
cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of
cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only
a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their
diagnosis in biological samples. |
| format |
Artigo |
| author |
Ribeiro, Ana Cl?udia da Silva |
| author2 |
Carvalho, Carlos Alberto Marques de Casseb, Samir Mansour Moraes Rodrigues, Sueli Guerreiro Vasconcelos, Pedro Fernando da Costa Carvalho, Val?ria Lima |
| author2Str |
Carvalho, Carlos Alberto Marques de Casseb, Samir Mansour Moraes Rodrigues, Sueli Guerreiro Vasconcelos, Pedro Fernando da Costa Carvalho, Val?ria Lima |
| title |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
| title_short |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
| title_full |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
| title_fullStr |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
| title_full_unstemmed |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
| title_sort |
infection profiles of mayaro virus and chikungunya virus in mammalian and mosquito cell lineages |
| publisher |
MS/SVS/Instituto Evandro Chagas |
| publishDate |
2019 |
| url |
http://patua.iec.gov.br//handle/iec/3883 |
| _version_ |
1717584511941738496 |
| score |
11.755432 |