New microsatellite markers for the neotropical malaria vector Anopheles nuneztovari sensu lato
Anopheles nuneztovari sensu lato consists of cryptic species and genetic lineages, one of which is an important human malaria vector in the northern part of South America. Population structure and evolutionary genetics studies may help in the definition and delimitation of the species and lineages w...
| Autor principal: | Cunha-Machado, Antônio Saulo |
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| Outros Autores: | Scarpassa, Vera Margarete |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Research
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14986 |
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oai:repositorio:1-14986 |
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oai:repositorio:1-14986 New microsatellite markers for the neotropical malaria vector Anopheles nuneztovari sensu lato Cunha-Machado, Antônio Saulo Scarpassa, Vera Margarete Allele Anopheles Nuneztovari Gene Library Gene Locus Genetic Variability Heterozygosity Insect Genetics Microsatellite Marker Nonhuman Nucleotide Sequence Population Genetic Structure Animals Anopheles Dna Sequence Gene Frequency Gene Linkage Disequilibrium Gene Vector Polymorphism, Genetic Genetics Genotype Human Malaria Molecular Genetics Polymerase Chain Reaction Transmission Anopheles Nuneztovari Microsatellite Dna Alleles Animal Anopheles Gene Frequency Genetic Vectors Genotype Humans Linkage Disequilibrium Malaria Microsatellite Repeats Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Sequence Analysis, Dna Anopheles nuneztovari sensu lato consists of cryptic species and genetic lineages, one of which is an important human malaria vector in the northern part of South America. Population structure and evolutionary genetics studies may help in the definition and delimitation of the species and lineages within this species complex, which is relevant information for organizations involved in malaria control efforts. In this study, 10 new microsatellite markers were isolated from 2 repeat-enriched genomic libraries of A. nuneztovari s.l. and were characterized in 37-48 mosquitoes of this species. All loci were highly polymorphic and encompassed 5-25 alleles per locus. The observed (HO) and expected (HE) heterozygosities ranged from 0.354 to 0.866 and from 0.613 to 0.932, respectively. Six of the 10 new loci showed significant deviations from the Hardy-Weinberg equilibrium, and no linkage disequilibrium was detected. The loci described in this study were more polymorphic than the 18 previously characterized loci and appear to be promising markers for use in investigating the fine-scale population genetic structure and the boundaries of the cryptic species and lineages within the A. nuneztovari complex. © FUNPEC-RP. 2020-05-07T14:00:20Z 2020-05-07T14:00:20Z 2014 Artigo https://repositorio.inpa.gov.br/handle/1/14986 10.4238/2014.October.27.26 en Volume 13, Número 4, Pags. 8856-8861 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Allele Anopheles Nuneztovari Gene Library Gene Locus Genetic Variability Heterozygosity Insect Genetics Microsatellite Marker Nonhuman Nucleotide Sequence Population Genetic Structure Animals Anopheles Dna Sequence Gene Frequency Gene Linkage Disequilibrium Gene Vector Polymorphism, Genetic Genetics Genotype Human Malaria Molecular Genetics Polymerase Chain Reaction Transmission Anopheles Nuneztovari Microsatellite Dna Alleles Animal Anopheles Gene Frequency Genetic Vectors Genotype Humans Linkage Disequilibrium Malaria Microsatellite Repeats Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Sequence Analysis, Dna |
| spellingShingle |
Allele Anopheles Nuneztovari Gene Library Gene Locus Genetic Variability Heterozygosity Insect Genetics Microsatellite Marker Nonhuman Nucleotide Sequence Population Genetic Structure Animals Anopheles Dna Sequence Gene Frequency Gene Linkage Disequilibrium Gene Vector Polymorphism, Genetic Genetics Genotype Human Malaria Molecular Genetics Polymerase Chain Reaction Transmission Anopheles Nuneztovari Microsatellite Dna Alleles Animal Anopheles Gene Frequency Genetic Vectors Genotype Humans Linkage Disequilibrium Malaria Microsatellite Repeats Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Sequence Analysis, Dna Cunha-Machado, Antônio Saulo New microsatellite markers for the neotropical malaria vector Anopheles nuneztovari sensu lato |
| topic_facet |
Allele Anopheles Nuneztovari Gene Library Gene Locus Genetic Variability Heterozygosity Insect Genetics Microsatellite Marker Nonhuman Nucleotide Sequence Population Genetic Structure Animals Anopheles Dna Sequence Gene Frequency Gene Linkage Disequilibrium Gene Vector Polymorphism, Genetic Genetics Genotype Human Malaria Molecular Genetics Polymerase Chain Reaction Transmission Anopheles Nuneztovari Microsatellite Dna Alleles Animal Anopheles Gene Frequency Genetic Vectors Genotype Humans Linkage Disequilibrium Malaria Microsatellite Repeats Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Sequence Analysis, Dna |
| description |
Anopheles nuneztovari sensu lato consists of cryptic species and genetic lineages, one of which is an important human malaria vector in the northern part of South America. Population structure and evolutionary genetics studies may help in the definition and delimitation of the species and lineages within this species complex, which is relevant information for organizations involved in malaria control efforts. In this study, 10 new microsatellite markers were isolated from 2 repeat-enriched genomic libraries of A. nuneztovari s.l. and were characterized in 37-48 mosquitoes of this species. All loci were highly polymorphic and encompassed 5-25 alleles per locus. The observed (HO) and expected (HE) heterozygosities ranged from 0.354 to 0.866 and from 0.613 to 0.932, respectively. Six of the 10 new loci showed significant deviations from the Hardy-Weinberg equilibrium, and no linkage disequilibrium was detected. The loci described in this study were more polymorphic than the 18 previously characterized loci and appear to be promising markers for use in investigating the fine-scale population genetic structure and the boundaries of the cryptic species and lineages within the A. nuneztovari complex. © FUNPEC-RP. |
| format |
Artigo |
| author |
Cunha-Machado, Antônio Saulo |
| author2 |
Scarpassa, Vera Margarete |
| author2Str |
Scarpassa, Vera Margarete |
| title |
New microsatellite markers for the neotropical malaria vector Anopheles nuneztovari sensu lato |
| title_short |
New microsatellite markers for the neotropical malaria vector Anopheles nuneztovari sensu lato |
| title_full |
New microsatellite markers for the neotropical malaria vector Anopheles nuneztovari sensu lato |
| title_fullStr |
New microsatellite markers for the neotropical malaria vector Anopheles nuneztovari sensu lato |
| title_full_unstemmed |
New microsatellite markers for the neotropical malaria vector Anopheles nuneztovari sensu lato |
| title_sort |
new microsatellite markers for the neotropical malaria vector anopheles nuneztovari sensu lato |
| publisher |
Genetics and Molecular Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14986 |
| _version_ |
1787144321511718912 |
| score |
11.653393 |