Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species
Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250...
| Autor principal: | Cruz-Hernández, Pedro |
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| Outros Autores: | Batista, Jacqueline da Silva, Formiga, Kyara M., Rafael, Míriam Silva, Tadei, Wanderli Pedro, Santos, Joselita Maria M. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Research
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14993 |
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oai:repositorio:1-14993 |
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oai:repositorio:1-14993 Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species Cruz-Hernández, Pedro Batista, Jacqueline da Silva Formiga, Kyara M. Rafael, Míriam Silva Tadei, Wanderli Pedro Santos, Joselita Maria M. Genomic Dna Allele Anopheles Anopheles Triannulatus Brasil Dna Hybridization Gene Amplification Gene Locus Genetic Linkage Heterozygosity Microsatellite Marker Nonhuman Nucleotide Sequence Polymorphic Dna Microsatellite Locus Genetics, Population Alleles Animal Anopheles Brasil Dna Genetics, Population Humans Linkage Disequilibrium Microsatellite Repeats Polymorphism, Genetic Species Specificity Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP. 2020-05-07T14:00:23Z 2020-05-07T14:00:23Z 2013 Artigo https://repositorio.inpa.gov.br/handle/1/14993 10.4238/2013.March.13.11 en Volume 12, Número 3, Pags. 3088-3092 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Genomic Dna Allele Anopheles Anopheles Triannulatus Brasil Dna Hybridization Gene Amplification Gene Locus Genetic Linkage Heterozygosity Microsatellite Marker Nonhuman Nucleotide Sequence Polymorphic Dna Microsatellite Locus Genetics, Population Alleles Animal Anopheles Brasil Dna Genetics, Population Humans Linkage Disequilibrium Microsatellite Repeats Polymorphism, Genetic Species Specificity |
| spellingShingle |
Genomic Dna Allele Anopheles Anopheles Triannulatus Brasil Dna Hybridization Gene Amplification Gene Locus Genetic Linkage Heterozygosity Microsatellite Marker Nonhuman Nucleotide Sequence Polymorphic Dna Microsatellite Locus Genetics, Population Alleles Animal Anopheles Brasil Dna Genetics, Population Humans Linkage Disequilibrium Microsatellite Repeats Polymorphism, Genetic Species Specificity Cruz-Hernández, Pedro Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
| topic_facet |
Genomic Dna Allele Anopheles Anopheles Triannulatus Brasil Dna Hybridization Gene Amplification Gene Locus Genetic Linkage Heterozygosity Microsatellite Marker Nonhuman Nucleotide Sequence Polymorphic Dna Microsatellite Locus Genetics, Population Alleles Animal Anopheles Brasil Dna Genetics, Population Humans Linkage Disequilibrium Microsatellite Repeats Polymorphism, Genetic Species Specificity |
| description |
Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP. |
| format |
Artigo |
| author |
Cruz-Hernández, Pedro |
| author2 |
Batista, Jacqueline da Silva Formiga, Kyara M. Rafael, Míriam Silva Tadei, Wanderli Pedro Santos, Joselita Maria M. |
| author2Str |
Batista, Jacqueline da Silva Formiga, Kyara M. Rafael, Míriam Silva Tadei, Wanderli Pedro Santos, Joselita Maria M. |
| title |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
| title_short |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
| title_full |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
| title_fullStr |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
| title_full_unstemmed |
Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species |
| title_sort |
isolation and characterization of polymorphic dna microsatellite loci for anopheles triannulatus sensu lato (diptera: culicidae) and cross-amplification in congeneric species |
| publisher |
Genetics and Molecular Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14993 |
| _version_ |
1787145415531954176 |
| score |
11.653393 |