Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.

Guimarães-Marques, Giselle Moura; Batista, Jacqueline da Silva; Guimarães, H. M.; Naice-Daou, M. V.; Lima, M. P.; Formiga, Kyara M.; Santos, Joselita Maria M.; Lima, A. C.; Rafael, Míriam Silva

Artigo

Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated a...

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Autor principal:	Guimarães-Marques, Giselle Moura
Outros Autores:	Batista, Jacqueline da Silva, Guimarães, H. M., Naice-Daou, M. V., Lima, M. P., Formiga, Kyara M., Santos, Joselita Maria M., Lima, A. C., Rafael, Míriam Silva
Grau:	Artigo
Idioma:	English
Publicado em:	Genetics and Molecular Research 2020
Assuntos:	Microsatellite Dna Animals Anopheles Disease Carrier Dna Sequence Gene Gene Locus Genetics Genotype Polymerase Chain Reaction Species Difference Animal Anopheles Genes, Insect Genetic Loci Genotype Insect Vectors Microsatellite Repeats Polymerase Chain Reaction Sequence Analysis, Dna Species Specificity
Acesso em linha:	https://repositorio.inpa.gov.br/handle/1/14997

id	oai:repositorio:1-14997
recordtype	dspace
spelling	oai:repositorio:1-14997 Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species. Guimarães-Marques, Giselle Moura Batista, Jacqueline da Silva Guimarães, H. M. Naice-Daou, M. V. Lima, M. P. Formiga, Kyara M. Santos, Joselita Maria M. Lima, A. C. Rafael, Míriam Silva Microsatellite Dna Animals Anopheles Disease Carrier Dna Sequence Gene Gene Locus Genetics Genotype Polymerase Chain Reaction Species Difference Animal Anopheles Genes, Insect Genetic Loci Genotype Insect Vectors Microsatellite Repeats Polymerase Chain Reaction Sequence Analysis, Dna Species Specificity The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species. 2020-05-07T14:00:25Z 2020-05-07T14:00:25Z 2012 Artigo https://repositorio.inpa.gov.br/handle/1/14997 10.4238/2012.December.6.3 en Volume 11, Número 4, Pags. 4198-4203 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Research
institution	Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection	INPA-RI
language	English
topic	Microsatellite Dna Animals Anopheles Disease Carrier Dna Sequence Gene Gene Locus Genetics Genotype Polymerase Chain Reaction Species Difference Animal Anopheles Genes, Insect Genetic Loci Genotype Insect Vectors Microsatellite Repeats Polymerase Chain Reaction Sequence Analysis, Dna Species Specificity
spellingShingle	Microsatellite Dna Animals Anopheles Disease Carrier Dna Sequence Gene Gene Locus Genetics Genotype Polymerase Chain Reaction Species Difference Animal Anopheles Genes, Insect Genetic Loci Genotype Insect Vectors Microsatellite Repeats Polymerase Chain Reaction Sequence Analysis, Dna Species Specificity Guimarães-Marques, Giselle Moura Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
topic_facet	Microsatellite Dna Animals Anopheles Disease Carrier Dna Sequence Gene Gene Locus Genetics Genotype Polymerase Chain Reaction Species Difference Animal Anopheles Genes, Insect Genetic Loci Genotype Insect Vectors Microsatellite Repeats Polymerase Chain Reaction Sequence Analysis, Dna Species Specificity
description	The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species.
format	Artigo
author	Guimarães-Marques, Giselle Moura
author2	Batista, Jacqueline da Silva Guimarães, H. M. Naice-Daou, M. V. Lima, M. P. Formiga, Kyara M. Santos, Joselita Maria M. Lima, A. C. Rafael, Míriam Silva
author2Str	Batista, Jacqueline da Silva Guimarães, H. M. Naice-Daou, M. V. Lima, M. P. Formiga, Kyara M. Santos, Joselita Maria M. Lima, A. C. Rafael, Míriam Silva
title	Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
title_short	Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
title_full	Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
title_fullStr	Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
title_full_unstemmed	Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
title_sort	isolation and characterization of 25 microsatellite dna loci for anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
publisher	Genetics and Molecular Research
publishDate	2020
url	https://repositorio.inpa.gov.br/handle/1/14997
_version_	1787141129943121920
score	11.653393

Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.

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